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(a)定点偶联特异性寡核苷酸探针的一对抗体特异性识别待测抗原后，形成一个三明治双抗体夹心结构
(b)抗体上偶联的寡核苷酸靠近，在酶的作用下可连接成可扩增的双链DNA,蛋白信号转换成核酸信号
(c)经qPCR扩增后，Ct值与待测抗原浓度呈负相关，通过标准曲线即可计算代测样本中的目标抗原浓度。
试剂盒试剂组分，组分有颜色编码，方便使用。
试剂盒外包装和各试剂包装
产品操作视频
产品属性/ Product Details
| 产品名称 | 人肿瘤坏死因子α(TNF-α) VeriQuant 免疫分析试剂盒 | 反应种属 | Human |
| 基因别名 | DIF, IMD127, TNF, TNF-alpha, TNFA, TNFSF2, TNLG1F | 基因ID | 7124 |
| Uniprot ID | P01375 | 蛋白别名 | DIF, TNF-alpha, TNFA, TNFSF2,TNF,TNFa |
| 检测方法 | Proximity-based Immunoassay | 灵敏度 | 0.063pg/mL |
| 检测仪器 | qPCR Instruments | 检测范围 | 0.256-4000 pg/mL |
| 检测样本类型/体积 | Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other biological fluids；Sample Volume=1~5μL | ||
| 检测总时间 | 2 hr | 手工操作时间 | 20 min |
| 运输条件 | 超低温相变材料 | 储存温度 | -20°C |
| 均相(无需洗涤) | Yes | 标签或染料 | FAM™ |
| 关于本试剂盒 | 人 TNF-a VeriQuant免疫分析试剂盒旨在对微量样本中的人 TNF-a 蛋白进行高灵敏度的定量测量。该测定采用自研ReedLytix偶联体系和邻近连接技术 (Proximity Ligation Assay) ，将高亲和力抗体对待测物中抗原的特异性识别能力与qPCR 的指数级信号放大能力相结合，打造了一个强大的下一代蛋白定量平台。简单的操作流程，仅需 2 小时即可从样品到结果。从PCR仪上导出的结果可以在官网直接进行计算（https://www.reedbio.cn/list/122.html） ①超敏：全系产品都达到飞克级灵敏度 ②宽范围：产品检测范围宽度达到6个数量级 ③微量：最少样本量仅需1μL ④快速：全程2H（手工操作20min），无需洗板 ⑤ALL IN ONE:可同时替代传统、高敏、微量、一步法、宽范围等系列ELISA Kits |
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| 靶标信息 | TNF alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophage and bind to its receptors, TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF alpha is involved in the regulation of immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS-PAGE under non-reducing conditions. Knockout studies in mice also suggested the neuroprotective function of TNF alpha, and has been observed to causes tumor necrosis when injected into tumor-bearing mice. Other functions of TNF-alpha include its role in the immune response to bacterial, viral, parasitic and certain fungal infections, as well as its role in the necrosis of specific tumors. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon-gamma in its cytotoxicity. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. | ||