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(a)定点偶联特异性寡核苷酸探针的一对抗体特异性识别待测抗原后，形成一个三明治双抗体夹心结构
(b)抗体上偶联的寡核苷酸靠近，在酶的作用下可连接成可扩增的双链DNA,蛋白信号转换成核酸信号
(c)经qPCR扩增后，Ct值与待测抗原浓度呈负相关，通过标准曲线即可计算代测样本中的目标抗原浓度。
试剂盒试剂组分，组分有颜色编码，方便使用。
试剂盒外包装和各试剂包装
产品操作视频
产品属性/ Product Details
| 产品名称 | 大鼠肿瘤坏死因子α(TNF-α) VeriQuant 免疫分析试剂盒 | 反应种属 | Rat |
| 基因别名 | DIF, Tnf, TNF-a, TNF-alpha, Tnfa, TNFalpha, Tnfsf1a, TNFSF2 | 基因ID | 24835 |
| Uniprot ID | P16599 | 蛋白别名 | APC1 protein; Cachectin; Cachetin; DIF; TNF; TNF, monocyte-derived; TNFA; TNF-A; TNFalpha; TNF-alpha; TNF-alphacachectin; TNFATNF, macrophage-derived; TNFG1F; TNFSF1A; TNFSF2; TNFSF2TNF superfamily, member 2; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; Tumor necrosis factor ligand superfamily member 2; tumor necrosis factor; tumor necrosis factor-alpha |
| 检测方法 | Proximity-based Immunoassay | 灵敏度 | 0.101pg/mL |
| 检测仪器 | qPCR Instruments | 检测范围 | 0.32-5000 pg/mL |
| 检测样本类型/体积 | Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other biological fluids；Sample Volume=1~5μL | ||
| 检测总时间 | 2 hr | 手工操作时间 | 20 min |
| 运输条件 | 超低温相变材料 | 储存温度 | -20°C |
| 均相(无需洗涤) | Yes | 标签或染料 | FAM™ |
| 关于本试剂盒 | 大鼠 TNF-a VeriQuant免疫分析试剂盒旨在对微量样本中的大鼠 TNF-a 蛋白进行高灵敏度的定量测量。该测定采用自研ReedLytix偶联体系和邻近连接技术 (Proximity Ligation Assay) ，将高亲和力抗体对待测物中抗原的特异性识别能力与qPCR 的指数级信号放大能力相结合，打造了一个强大的下一代蛋白定量平台。简单的操作流程，仅需 2 小时即可从样品到结果。从PCR仪上导出的结果可以在官网直接进行计算（https://www.reedbio.cn/list/122.html） ①超敏：全系产品都达到飞克级灵敏度 ②宽范围：产品检测范围宽度达到6个数量级 ③微量：最少样本量仅需1μL ④快速：全程2H（手工操作20min），无需洗板 ⑤ALL IN ONE:可同时替代传统、高敏、微量、一步法、宽范围等系列ELISA Kits |
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| 靶标信息 | Enables tumor necrosis factor receptor binding activity. Involved in several processes, including negative regulation of transport; positive regulation of cell communication; and positive regulation of macromolecule biosynthetic process. Acts upstream of or within positive regulation of interleukin-6 production. Located in external side of plasma membrane; extracellular space; and neuronal cell body. Is active in extracellular region. Used to study several diseases, including cerebrovascular disease (multiple); liver disease (multiple); peptic esophagitis; perinatal necrotizing enterocolitis; and periventricular leukomalacia. Biomarker of several diseases, including artery disease (multiple); brain disease (multiple); congestive heart failure (multiple); liver disease (multiple); and lung disease (multiple). Human ortholog(s) of this gene implicated in several diseases, including autoimmune disease (multiple); eye disease (multiple); liver disease (multiple); lung disease (multiple); and skin disease (multiple). Orthologous to human TNF (tumor necrosis factor). | ||