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(a)定点偶联特异性寡核苷酸探针的一对抗体特异性识别待测抗原后，形成一个三明治双抗体夹心结构
(b)抗体上偶联的寡核苷酸靠近，在酶的作用下可连接成可扩增的双链DNA,蛋白信号转换成核酸信号
(c)经qPCR扩增后，Ct值与待测抗原浓度呈负相关，通过标准曲线即可计算代测样本中的目标抗原浓度。
试剂盒试剂组分，组分有颜色编码，方便使用。
试剂盒外包装和各试剂包装
产品操作视频
产品属性/ Product Details
| 产品名称 | 人白介素1β(IL-1β) VeriQuant 免疫分析试剂盒 | 反应种属 | Human |
| 基因别名 | IL-1, IL1-BETA, IL1B, IL1beta, IL1F2 | 基因ID | 3553 |
| Uniprot ID | P01584 | 蛋白别名 | IL1B, IL1-BETA, IL1F2, catabolin, IL1b, IL-1B, IL-1b,Catabolin, IL-1 beta, Hematopoietin 1 (H1), IFN beta inducing factor, il 1b, IL 1β, IL1 B, IL1β, Osteoclast activating factor (OAF), Pro interleukin 1 beta, ILN, Interleukin, Interleukin 1 beta precursor, Interleukin1 beta, Interleukin-1b, LAF, IL1B, interleukin 1 precursor polypeptide, interleukin 1beta, Interleukin-1 beta, preinterleukin 1 beta, pro-interleukin-1-beta, unnamed protein product |
| 检测方法 | Proximity-based Immunoassay | 灵敏度 | 0.077pg/mL |
| 检测仪器 | qPCR Instruments | 检测范围 | 0.32-5000 pg/mL |
| 检测样本类型/体积 | Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other biological fluids；Sample Volume=1~5μL | ||
| 检测总时间 | 2 hr | 手工操作时间 | 20 min |
| 运输条件 | 超低温相变材料 | 储存温度 | -20°C |
| 均相(无需洗涤) | Yes | 标签或染料 | FAM™ |
| 关于本试剂盒 | 人 IL-1β VeriQuant免疫分析试剂盒旨在对微量样本中的人 IL-1β 蛋白进行高灵敏度的定量测量。该测定采用自研ReedLytix偶联体系和邻近连接技术 (Proximity Ligation Assay) ，将高亲和力抗体对待测物中抗原的特异性识别能力与qPCR 的指数级信号放大能力相结合，打造了一个强大的下一代蛋白定量平台。简单的操作流程，仅需 2 小时即可从样品到结果。从PCR仪上导出的结果可以在官网直接进行计算（https://www.reedbio.cn/list/122.html） ①超敏：全系产品都达到飞克级灵敏度 ②宽范围：产品检测范围宽度达到6个数量级 ③微量：最少样本量仅需1μL ④快速：全程2H（手工操作20min），无需洗板 ⑤ALL IN ONE:可同时替代传统、高敏、微量、一步法、宽范围等系列ELISA Kits |
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| 靶标信息 | Interleukin-1 beta (IL-1 beta) is a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells. IL-1 beta is synthesized in response to inflammatory stimuli as a 31 kDa inactive pro-form that accumulates in the cytosol. Cleavage of pro-IL-1 beta into the active 17 kDa protein requires the activation of inflammasomes, which are multi-protein complexes that respond to pathogens, stress conditions, and other danger signals. Inflammasome activation triggers the processing of the caspase-1 precursor into its active form, which in turn cleaves pro-IL-1 beta. IL-1 beta lacks a signal sequence peptide for classical ER/Golgi pathway and is secreted alongside caspase-1 via an alternate and incompletely understood mechanism. Although IL-1 beta is most often secreted in its active form, secretion of the uncleaved protein may be detectable under some biological conditions. IL-1 beta signals through two receptors, IL-1RI and IL-1RII, both of which are shared with IL-1 alpha. IL-1 beta activity can be moderated by IL-1 Receptor Antagonist (IL-1RA), a protein produced by many cell types that blocks receptor binding through competitive inhibition. IL-1 beta play an important role in innate host defense by triggering the production of other proinflammatory cytokines in target cells and initiating acute-phase responses to infection and injury. Elevated levels of IL-1 beta have been associated with many chronic inflammatory conditions IL-1 beta neutralizing antibodies potential therapeutic value. | ||