大鼠白介素6(IL-6)酶联免疫吸附测定试剂盒-瑞迪生物科技（武汉）有限公司

产品名称	Rat IL-6(Interleukin 6) ELISA Kit	反应种属	Rat
Uniprot ID	P20607	别称	IL-6;IL 6;IL6;Interleukin-6;Interleukin 6;Interleukin6;B-cell differentiation factor, B-cell stimulatory factor 2, BSF2, BSF-2, CDF, CTL differentiation factor , HSF, hybridoma growth factor, IFNB2, IFN-beta-2, IL6, IL-6, Interferon beta-2, interleukin 6 (interferon, beta 2), interleukin BSF-2, interleukin-6, MGI-2A;Hybridoma growth factor;Interferon beta-2;IFNB 2;IFNB-2;IFN-B2
检测方法	Sandwich	灵敏度	4.75 pg/mL
标准品	500pg/mL	检测范围	7.81-500pg/mL
样品类型	Serum, Plasma, Tissue homogenate and Other biological samples；Sample Volume=100μL
检测时间	3.5H	研究领域	Tumor immunity;Endocrinology;Hormone metabolism;
测试原理	This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat IL-6. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat IL-6 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat IL-6, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat IL-6. You can calculate the concentration of Rat IL-6 in the samples by comparing the OD of the samples to the standard curve.

技术数据/ Technical Data

由于实验操作条件的不同(如操作者、移液技术、洗板技术和稳定条件等)，标准曲线的OD值会有所差异。以下数据和曲线仅供参考，实验者需根据自己的实验建立标准曲线。

(pg/mL)	OD	Corrected
500.00	2.392	2.340
250.00	1.745	1.693
125.00	0.873	0.821
62.50	0.473	0.421
31.25	0.264	0.212
15.62	0.131	0.079
7.81	0.095	0.043
0.00	0.052	0.000

精密度

板内精密度:低浓度样本,中浓度样本和高浓度样本分别在1块板子上检测20次。

板间精密度:低浓度样本,中浓度样本和高浓度样本分别在3块板子上检测20次。

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean(pg/mL)	13.51	191.69	357.43	14.35	164.67	355.13
Standard deviation	1.19	7.21	17.16	1.00	8.04	10.81
C V (%)	7.40	5.55	4.79	7.54	3.80	2.16

回收率

分别往5个不同样本中添加已知浓度的目标蛋白，做回收实验，得出回收率范围和平均回收率。

Sample Type	Range(%)	Average Recovery(%)
Serum (n=8)	83-97	90
EDTA plasma (n=8)	87-95	91
Cell culture media (n=8)	81-95	88

线性

分别往5个样本中添加已知浓度的目标蛋白，做回收实验，得出回收率范围及平均回收率。将5个样本分别稀释2倍，4倍，8倍，16倍做回收实验，得出回收率范围及平均回收率。

		Serum (n=5)	EDTA plasma (n=5)	Cell culture media (n=5)
1:2	Range (%)	93-102	86-96	87-96
1:2	Average (%)	96	95	92
1:4	Range (%)	81-96	93-104	85-97
1:4	Average (%)	89	98	96
1:8	Range (%)	93-106	89-97	85-94
1:8	Average (%)	96	93	88
1:16	Range (%)	82-95	85-94	87-96
1:16	Average (%)	88	93	93

检测流程/ Assay Procedures